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Cellosaurus WTC-mEGFP-ACTB-cl184 (CVCL_JM16)

[Text version]
Cell line name WTC-mEGFP-ACTB-cl184
Synonyms ACTB-GFP WTC; AICS-0016-184; AICS0016; AICS-0016; ACTB1 cl. 184; AICSi002-A-3; UCSFi001-A-3
Accession CVCL_JM16
Resource Identification Initiative To cite this cell line use: WTC-mEGFP-ACTB-cl184 (RRID:CVCL_JM16)
Comments Part of: Allen Cell Collection.
Population: Japanese.
Characteristics: Using CRISPR/Cas9 ACTB has been endogenously tagged in one allele at the N-terminus with mEGFP.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Omics: Deep exome analysis.
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Web pages https://www.allencell.org/cell-catalog.html
https://www.allencell.org/genomics.html
Publications

PubMed=28814507; DOI=10.1091/mbc.E17-03-0209; PMCID=PMC5638588
Roberts B., Haupt A., Tucker A., Grancharova T., Arakaki J., Fuqua M.A., Nelson A.M., Hookway C., Ludmann S.A., Mueller I.A., Yang R.-A., Horwitz R., Rafelski S.M., Gunawardane R.N.
Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization.
Mol. Biol. Cell 28:2854-2874(2017)

PubMed=34019794; DOI=10.1016/j.cell.2021.04.034
Hofbauer P., Jahnel S.M., Papai N., Giesshammer M., Deyett A., Schmidt C., Penc M., Tavernini K., Grdseloff N., Meledeth C., Ginistrelli L.C., Ctortecka C., Salic S., Novatchkova M., Mendjan S.
Cardioids reveal self-organizing principles of human cardiogenesis.
Cell 184:3299-3317.e22(2021)

Cross-references
Cell line collections (Providers) Coriell; AICS-0016
Cell line databases/resources hPSCreg; UCSFi001-A-3
Biological sample resources BioSamples; SAMEA6298548
Encyclopedic resources Wikidata; Q54994540
Entry history
Entry creation15-May-2017
Last entry update10-Sep-2024
Version number14