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Cellosaurus POLK CD (CVCL_JH01)

[Text version]
Cell line name POLK CD
Synonyms Nalm-6-MSH+/TK+/-/POLK D198A/D199A
Accession CVCL_JH01
Resource Identification Initiative To cite this cell line use: POLK CD (RRID:CVCL_JH01)
Comments Population: Caucasian.
Knockout cell: Method=Homologous recombination; HGNC; 11830; TK1 (Note=1 of 2 alleles).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 7325; MSH2 (Note=Exons 9 to 16).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene fusion; HGNC; 50800; DUX4 + HGNC; 5477; IGH; Name(s)=IGH-DUX4; Note=The translocation occurs in the silenced IGH allele (from parent cell line).
  • Mutation; HGNC; 3236; EGFR; Simple; p.Arg1068Ter (c.3202C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Ala146Thr (c.436G>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9183; POLK; Simple_edited; p.Asp198Ala; Zygosity=Unspecified; Note=Loss of activity. By homologous recombination (PubMed=24461735).
  • Mutation; HGNC; 9183; POLK; Simple_edited; p.Asp199Ala; Zygosity=Unspecified; Note=Loss of activity. By homologous recombination (PubMed=24461735).
  • Mutation; HGNC; 9864; RARA; Simple; p.Gln45Ter (c.133C>T); ClinVar=VCV000393019; Zygosity=Heterozygous (from parent cell line).
Disease Adult B acute lymphoblastic leukemia (NCIt: C9143)
Precursor B-cell acute lymphoblastic leukemia (ORDO: Orphanet_99860)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_UD71 (Nalm-6-MSH+/TK+/-)
Sex of cell Male
Age at sampling 19Y
Category Cancer cell line
STR profile Source(s): JCRB=JCRB1524

Markers:
AmelogeninX,Y
CSF1PO15
D5S81811,12
D7S8208,12
D13S31710,14
D16S53910,11
TH018,9
TPOX8,10
vWA15,16

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Publications

PubMed=24461735; DOI=10.1016/j.dnarep.2013.12.008
Sassa A., Suzuki T., Kanemaru Y., Niimi N., Fujimoto H., Katafuchi A., Gruz P., Yasui M., Gupta R.C., Johnson F., Ohta T., Honma M., Adachi N., Nohmi T.
In vivo evidence that phenylalanine 171 acts as a molecular brake for translesion DNA synthesis across benzo[a]pyrene DNA adducts by human DNA polymerase kappa.
DNA Repair 15:21-28(2014)

Cross-references
Cell line collections (Providers) JCRB; JCRB1524
Cell line databases/resources cancercelllines; CVCL_JH01
Encyclopedic resources Wikidata; Q54947710
Entry history
Entry creation15-May-2017
Last entry update10-Sep-2024
Version number16