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Cellosaurus NtG20.i7 (CVCL_JA65)

[Text version]
Cell line name NtG20.i7
Accession CVCL_JA65
Resource Identification Initiative To cite this cell line use: NtG20.i7 (RRID:CVCL_JA65)
Comments Population: Caucasian.
Virology: Supports hepatitis B virus (HBV) replication for up to 35 days with production of infectious HBV in the culture supernatant.
Knockout cell: Method=shRNA knockdown; HGNC; HGNC:29233; MAVS.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:10905; SLC10A1.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Hepatoblastoma (NCIt: C3728)
Hepatoblastoma (ORDO: Orphanet_449)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0027 (Hep-G2)
Sex of cell Male
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=28338936; DOI=10.1093/intimm/dxx012
Yao W.-L., Ikeda S., Tsukamoto Y., Shindo K., Otakaki Y., Qin M., Iwasawa Y., Takeuchi F., Kaname Y., Chou Y.-C., Chang C.-M., Watashi K., Wakita T., Noda T., Kato H., Fujita T.
Establishment of a human hepatocellular cell line capable of maintaining long-term replication of hepatitis B virus.
Int. Immunol. 29:109-120(2017)

Cross-references
Cell line databases/resources cancercelllines; CVCL_JA65
Encyclopedic resources Wikidata; Q54931114
Entry history
Entry creation15-May-2017
Last entry update19-Dec-2024
Version number17