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Cellosaurus TWNT-1 (CVCL_J364)

[Text version]
Cell line name TWNT-1
Accession CVCL_J364
Resource Identification Initiative To cite this cell line use: TWNT-1 (RRID:CVCL_J364)
Comments Group: Patented cell line.
Registration: International Depositary Authority, Japanese National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology; FERM BP-7843.
Population: Japanese.
Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the TERT gene thus reverting the cells to a non-immortal phenotype.
Characteristics: Transduced with a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatic stellate cell; CL=CL_0000632.
Disease Epithelioid hemangioendothelioma (NCIt: C3800)
Epithelioid hemangioendothelioma (ORDO: Orphanet_157791)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0390 (LI90)
Sex of cell Female
Age at sampling 55Y
Category Telomerase immortalized cell line
STR profile Source(s): JCRB=JCRB1582

Markers:
AmelogeninX
CSF1PO9,12
D5S81811,13
D7S82010,11
D13S31712
D16S5399,10
TH019
TPOX8,11
vWA16,17

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Publications

PubMed=12811248; DOI=10.1097/01.TP.0000064621.50907.A6
Watanabe T., Shibata N., Westerman K.A., Okitsu T., Allain J.-E., Sakaguchi M., Totsugawa T., Maruyama M., Matsumura T., Noguchi H., Yamamoto S., Hikida M., Ohmori A., Reth M.G., Weber A., Tanaka N., Leboulch P., Kobayashi N.
Establishment of immortalized human hepatic stellate scavenger cells to develop bioartificial livers.
Transplantation 75:1873-1880(2003)

Patent=US7521234
Kobayashi N., Leboulch P., Tanaka N., Fujiwara T., Totsugawa T.
Mammalian immortalized liver cell.
Patent number US7521234, 21-Apr-2009

Cross-references
Cell line collections (Providers) JCRB; JCRB1582
Encyclopedic resources Wikidata; Q54973428
Entry history
Entry creation11-Feb-2013
Last entry update19-Dec-2024
Version number20