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Cellosaurus Ras[V12]-wts[RNAi] (CVCL_IY71)

[Text version]
Cell line name Ras[V12]-wts[RNAi]
Synonyms Ras[v12];wts[RNAi]; WRR-1
Accession CVCL_IY71
Resource Identification Initiative To cite this cell line use: Ras[V12]-wts[RNAi] (RRID:CVCL_IY71)
Comments Group: Insect cell line.
Characteristics: Established from embryos carrying Act5C-GAL4, UAS-Ras[V12] and UAS-wts[RNAi] transposons.
Doubling time: 2 days (DGRC=189).
Genetic integration: Method=Transfection/transduction; Gene=FlyBase_Gene; FBgn0003205; Ras85D (Note=With p.Gly12Val).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04386; Yeast GAL4.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Sex of cell Male
Age at sampling Embryonic stage
Category Transformed cell line
Publications

PubMed=18670627; DOI=10.1371/journal.pgen.1000142; PMCID=PMC2474701
Simcox A.A., Mitra S., Truesdell S.L., Paul L., Chen T., Butchar J.P., Justiniano S.E.
Efficient genetic method for establishing Drosophila cell lines unlocks the potential to create lines of specific genotypes.
PLoS Genet. 4:e1000142.1-e1000142.12(2008)

PubMed=26772746; DOI=10.1534/g3.115.023366; PMCID=PMC4777130
Stoiber M., Celniker S.E., Cherbas L., Brown B., Cherbas P.
Diverse hormone response networks in 41 independent Drosophila cell lines.
G3 (Bethesda) 6:683-694(2016)

Cross-references
Cell line collections (Providers) DGRC; 189
Cell line databases/resources FlyBase_Cell_line; FBtc0000189
Encyclopedic resources Wikidata; Q54949226
Entry history
Entry creation15-May-2017
Last entry update10-Apr-2025
Version number11