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Cellosaurus WTC-mEGFP-LMNB1-cl210 (CVCL_IR32)

[Text version]
Cell line name WTC-mEGFP-LMNB1-cl210
Synonyms AICS-0013-210; AICS0013; AICS-0013; LMNB1 cl. 210; AICSi002-A-6; UCSFi001-A-6
Accession CVCL_IR32
Resource Identification Initiative To cite this cell line use: WTC-mEGFP-LMNB1-cl210 (RRID:CVCL_IR32)
Comments Part of: Allen Cell Collection.
Population: Japanese.
Characteristics: Using CRISPR/Cas9 LMNB1 has been endogenously tagged in one allele at the N-terminus with mEGFP.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Omics: Deep exome analysis.
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Web pages https://www.allencell.org/cell-catalog.html
https://www.allencell.org/genomics.html
Publications

PubMed=28814507; DOI=10.1091/mbc.E17-03-0209; PMCID=PMC5638588
Roberts B., Haupt A., Tucker A., Grancharova T., Arakaki J., Fuqua M.A., Nelson A.M., Hookway C., Ludmann S.A., Mueller I.A., Yang R.-A., Horwitz R., Rafelski S.M., Gunawardane R.N.
Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization.
Mol. Biol. Cell 28:2854-2874(2017)

PubMed=30199041; DOI=10.3791/58130; PMCID=PMC6231893
Haupt A., Grancharova T., Arakaki J., Fuqua M.A., Roberts B., Gunawardane R.N.
Endogenous protein tagging in human induced pluripotent stem cells using CRISPR/Cas9.
J. Vis. Exp. 138:e58130.1-e58130.9(2018)

Cross-references
Cell line collections (Providers) Coriell; AICS-0013
Cell line databases/resources hPSCreg; UCSFi001-A-6
Biological sample resources BioSamples; SAMEA6306320
Encyclopedic resources Wikidata; Q54994545
Entry history
Entry creation03-Mar-2017
Last entry update10-Sep-2024
Version number15