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Cellosaurus Lec4A (CVCL_IP62)

[Text version]
Cell line name Lec4A
Synonyms CHO-Lec4A; CHO Lec4A; Pro~Lec4.12-2; Pro~Lec4A.12-2; ProLec4A.12.2
Accession CVCL_IP62
Resource Identification Initiative To cite this cell line use: Lec4A (RRID:CVCL_IP62)
Comments Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Mutation; UniProtKB; P97259; Mgat5; Simple; p.Leu188Arg (c.563T>G); Zygosity=Homozygous; Note=Causes mistargeting of the protein to the ER (PubMed=8910328).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_4382 (CHO Pro-5)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=6295280; DOI=10.1016/0003-9861(82)90141-2
Stanley P.
Carbohydrate heterogeneity of vesicular stomatitis virus G glycoprotein allows localization of the defect in a glycosylation mutant of CHO cells.
Arch. Biochem. Biophys. 219:128-139(1982)

PubMed=2530238; DOI=10.1083/jcb.109.5.2089; PMCID=PMC2115852
Chaney W.G., Sundaram S., Friedman N., Stanley P.
The Lec4A CHO glycosylation mutant arises from miscompartmentalization of a Golgi glycosyltransferase.
J. Cell Biol. 109:2089-2096(1989)

PubMed=8910328; DOI=10.1074/jbc.271.44.27462
Weinstein J., Sundaram S., Wang X.-H., Delgado D., Basu R., Stanley P.
A point mutation causes mistargeting of Golgi GlcNAc-TV in the Lec4A Chinese hamster ovary glycosylation mutant.
J. Biol. Chem. 271:27462-27469(1996)

PubMed=24078077; DOI=10.1007/s00418-013-1146-1; PMCID=PMC4085668
Dong Z.-Z., Zuber C., Pierce M., Stanley P., Roth J.
Reduction in Golgi apparatus dimension in the absence of a residential protein, N-acetylglucosaminyltransferase V.
Histochem. Cell Biol. 141:153-164(2014)

Cross-references
Anatomy/cell type resources BTO; BTO_0005989
Encyclopedic resources Wikidata; Q54902426
Entry history
Entry creation03-Mar-2017
Last entry update19-Dec-2024
Version number12