Cell line name |
Lec4 |
Synonyms |
CHO-Lec4; CHO Lec4; Gat~Lec4.2D; Pro~5.Lec4.7B |
Accession |
CVCL_IP61 |
Resource Identification Initiative |
To cite this cell line use: Lec4 (RRID:CVCL_IP61) |
Comments |
Derived from site: In situ; Ovary; UBERON=UBERON_0000992. Cell type: Epithelial cell of ovary; CL=CL_2000064. |
Sequence variations |
- Mutation; UniProtKB; P97259; Mgat5; Simple; p.Asp135Valfs*22; Zygosity=Unspecified; Note=Truncated protein lacking enzymatic activity (PubMed=8910328).
- Mutation; UniProtKB; P97259; Mgat5; Simple; p.Ile598fs; Zygosity=Unspecified; Note=Truncated protein lacking enzymatic activity (PubMed=8910328).
|
Species of origin |
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
(NCBI Taxonomy: 10029) |
Hierarchy |
Parent: CVCL_4382 (CHO Pro-5) |
Sex of cell |
Female |
Age at sampling |
Adult |
Category |
Spontaneously immortalized cell line |
Publications | PubMed=6295280; DOI=10.1016/0003-9861(82)90141-2 Stanley P. Carbohydrate heterogeneity of vesicular stomatitis virus G glycoprotein allows localization of the defect in a glycosylation mutant of CHO cells. Arch. Biochem. Biophys. 219:128-139(1982) PubMed=6623313; DOI=10.1007/BF01574260 Stanley P. Lectin-resistant CHO cells: selection of new mutant phenotypes. Somatic Cell Genet. 9:593-608(1983) PubMed=2530238; DOI=10.1083/jcb.109.5.2089; PMCID=PMC2115852 Chaney W.G., Sundaram S., Friedman N., Stanley P. The Lec4A CHO glycosylation mutant arises from miscompartmentalization of a Golgi glycosyltransferase. J. Cell Biol. 109:2089-2096(1989) PubMed=8910328; DOI=10.1074/jbc.271.44.27462 Weinstein J., Sundaram S., Wang X.-H., Delgado D., Basu R., Stanley P. A point mutation causes mistargeting of Golgi GlcNAc-TV in the Lec4A Chinese hamster ovary glycosylation mutant. J. Biol. Chem. 271:27462-27469(1996) PubMed=24078077; DOI=10.1007/s00418-013-1146-1; PMCID=PMC4085668 Dong Z.-Z., Zuber C., Pierce M., Stanley P., Roth J. Reduction in Golgi apparatus dimension in the absence of a residential protein, N-acetylglucosaminyltransferase V. Histochem. Cell Biol. 141:153-164(2014) |
Cross-references |
Anatomy/cell type resources |
BTO; BTO_0005988
|
Encyclopedic resources |
Wikidata; Q54902425
|
Entry history |
Entry creation | 03-Mar-2017 |
Last entry update | 19-Dec-2024 |
Version number | 12 |
---|