ID   HL-60/AMSA
AC   CVCL_IP18
DR   cancercelllines; CVCL_IP18
DR   Wikidata; Q54889801
RX   PubMed=1311390;
RX   PubMed=1651812;
RX   PubMed=2550442;
CC   Population: Caucasian.
CC   Selected for resistance to: ChEBI; CHEBI:2687; Amsacrine.
CC   Sequence variation: Gene deletion; HGNC; 11998; TP53; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11989; TOP2A; Simple; p.Arg487Lys (c.1460G>A); ClinVar=VCV000016772; Zygosity=Unspecified; Note=Acquired during resistance selection process (PubMed=1311390).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
DI   NCIt; C9154; Adult acute myeloid leukemia
DI   ORDO; Orphanet_519; Acute myeloid leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0002 ! HL-60
SX   Female
AG   36Y
CA   Cancer cell line
DT   Created: 03-03-17; Last updated: 05-10-23; Version: 17
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RX   PubMed=1311390; DOI=10.1016/0022-2836(92)90245-F;
RA   Lee M.-S., Wang J.C., Beran M.;
RT   "Two independent amsacrine-resistant human myeloid leukemia cell lines
RT   share an identical point mutation in the 170 kDa form of human
RT   topoisomerase II.";
RL   J. Mol. Biol. 223:837-843(1992).
//
RX   PubMed=1651812;
RA   Hinds M., Deisseroth K., Mayes J., Altschuler E., Jansen R.,
RA   Ledley F.D., Zwelling L.A.;
RT   "Identification of a point mutation in the topoisomerase II gene from
RT   a human leukemia cell line containing an amsacrine-resistant form of
RT   topoisomerase II.";
RL   Cancer Res. 51:4729-4731(1991).
//
RX   PubMed=2550442; DOI=10.1016/S0021-9258(19)84722-1;
RA   Zwelling L.A., Hinds M., Chan D., Mayes J., Sie K.L., Parker E.,
RA   Silberman L., Radcliffe A., Beran M., Blick M.;
RT   "Characterization of an amsacrine-resistant line of human leukemia
RT   cells. Evidence for a drug-resistant form of topoisomerase II.";
RL   J. Biol. Chem. 264:16411-16420(1989).
//