Cellosaurus HEK293-EBNA1-6E (CVCL_HF20)
| Cell line name | HEK293-EBNA1-6E |
|---|---|
| Synonyms | HEK 293EBNA1-6E; HEK293-6E; HEK2936E; 293-6E; 293FEt-clone 6E |
| Accession | CVCL_HF20 |
| Resource Identification Initiative | To cite this cell line use: HEK293-EBNA1-6E (RRID:CVCL_HF20) |
| Comments | Group: Patented cell line. Registration: International Depositary Authority, IDA of Canada (IDAC); 150305-01. Characteristics: The use of recombinant protein expression vectors containing the EBV oriP in cell lines stably expressing EBV's EBNA1 protein significantly increases recombinant protein yield. Characteristics: This cell line is transfected with EBNA1t, a truncated version of EBV EBNA1 lacking the Gly-Gly-Ala repeats region. It has an enhanced ability to produce recombinant protein compared to HEK293-EBNA (Cellosaurus=CVCL_6974). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03211; Epstein-Barr virus (strain B95-8) EBNA1 (Note=With del 101-324 = EBNA1t). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_6642 (HEK293-F) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Web pages | https://web.archive.org/web/20190105224206/http://www.nrc-cnrc.gc.ca/eng/solutions/licensing/10894.html |
| Publications | PubMed=21356792; DOI=10.1101/pdb.prot4976 Patent=US8551774 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q54882446 |
| Entry history | |
| Entry creation | 01-Dec-2016 |
| Last entry update | 10-Sep-2024 |
| Version number | 11 |