| Cell line name |
HCT 116 CDKN1A(-/-) |
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| Synonyms |
CDKN1A (-/-) HCT116; HCT116 p21-/-; 80S14 |
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| Accession |
CVCL_HD73 |
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| Resource Identification Initiative |
To cite this cell line use: HCT 116 CDKN1A(-/-) (RRID:CVCL_HD73) |
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| Comments |
Population: Caucasian.
Knockout cell: Method=Homologous recombination; HGNC; HGNC:1784; CDKN1A.
Caution: There are two clones of p21-/- cells that were created, 80S4 and 80S14. It is not clear which clone this cell line corresponds to, but as 80S14 was much more used than 80S4 we believe it is that clone.
Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
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| Sequence variations |
- Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
- Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Leu450Ter (c.1349delT) (p.Leu450fs) (c.1344delT); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
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| Disease |
Colon carcinoma (NCIt: C4910) |
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| Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
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| Hierarchy |
Parent: CVCL_0291 (HCT 116) |
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| Sex of cell |
Male |
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| Age at sampling |
48Y |
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| Category |
Cancer cell line |
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| Publications | PubMed=7585571
Waldman T., Kinzler K.W., Vogelstein B.
p21 is necessary for the p53-mediated G1 arrest in human cancer cells.
Cancer Res. 55:5187-5190(1995) PubMed=8756351; DOI=10.1101/gad.10.15.1945
Polyak K., Waldman T., He T.-C., Kinzler K.W., Vogelstein B.
Genetic determinants of p53-induced apoptosis and growth arrest.
Genes Dev. 10:1945-1952(1996) |
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| Cross-references |
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| Cell line collections (Providers) |
Horizon_Discovery; HD+R02-035
|
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| Cell line databases/resources |
cancercelllines; CVCL_HD73
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| Anatomy/cell type resources |
BTO; BTO_0006532
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| Encyclopedic resources |
Wikidata; Q54881926
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| Entry history |
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| Entry creation | 01-Dec-2016 |
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| Last entry update | 19-Dec-2024 |
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| Version number | 13 |
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