Cellosaurus IMR-90/GFPdeltaRaf-1[YY]:ER (CVCL_HA48)
| Cell line name | IMR-90/GFPdeltaRaf-1[YY]:ER |
|---|---|
| Accession | CVCL_HA48 |
| Resource Identification Initiative | To cite this cell line use: IMR-90/GFPdeltaRaf-1[YY]:ER (RRID:CVCL_HA48) |
| Comments | Population: Caucasian. Characteristics: Transfected with a construct that is composed of EGFP at the N-terminus, the catalytic domain of RAF1 (with p.Tyr340Asp and p.Tyr341Asp) in the middle and the hormone-binding domain of ESR1 at the C-terminus. Protein kinase activity is activated by estrogen or analogs such as 4-hydroxy-tamoxifen. Genetic integration: Method=Transfection/transduction; Gene=HGNC; 9829; RAF1 (Note=Catalytic domain with p.Tyr340Asp and p.Tyr341Asp). Genetic integration: Method=Transfection/transduction; Gene=HGNC; 3467; ESR1 (Note=Hormone-binding domain). Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Derived from site: In situ; Fetal lung; UBERON=UBERON_0005597. Cell type: Fibroblast of lung; CL=CL_0002553. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0347 (IMR-90) |
| Sex of cell | Female |
| Age at sampling | 16FW |
| Category | Finite cell line |
| Publications | PubMed=9765202; DOI=10.1101/gad.12.19.2997; PMCID=PMC317194 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q54897673 |
| Entry history | |
| Entry creation | 26-Sep-2016 |
| Last entry update | 10-Sep-2024 |
| Version number | 12 |