ID   FM3A tsFT210
AC   CVCL_H224
SY   tsFT210; FT210
DR   Wikidata; Q54835215
RX   PubMed=1475706;
RX   PubMed=1903331;
RX   PubMed=2208288;
RX   PubMed=2428648;
RX   PubMed=9043671;
CC   Characteristics: Temperature sensitive phenotype. Stop growing at 39 Celsius. Cells are arrested at the G2 phase at 39 Celsius (PubMed=2428648).
CC   Doubling time: 16 hours (PubMed=2428648).
CC   Sequence variation: Mutation; MGI; MGI:88351; Cdk1; Simple; p.Pro272Ser (c.814C>T); Zygosity=Homozygous (PubMed=1903331; PubMed=2208288).
CC   Transformant: ChEBI; CHEBI:21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
CC   Caution: An additional Cdk1 mutation p.Ile52Val (c.154A>G) was reported by PubMed=2208288 but was not found by PubMed=1903331.
CC   Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
CC   Breed/subspecies: C3H/He.
DI   NCIt; C21678; Malignant neoplasms of the mouse mammary gland
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_3869 ! FM3A
SX   Female
CA   Cancer cell line
DT   Created: 11-02-13; Last updated: 29-06-23; Version: 8
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RX   PubMed=1475706; DOI=10.1007/BF01233079;
RA   Yasuda H., Nakata T., Kamijo M., Honda R., Nakamura M.,
RA   Ninomiya-Tsuji J., Yamashita M., Nagahama Y., Ohba Y.;
RT   "Cyclin-dependent kinase 2 (cdk2) in the murine Cdc2 kinase TS
RT   mutant.";
RL   Somat. Cell Mol. Genet. 18:403-408(1992).
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RX   PubMed=1903331; DOI=10.1247/csf.16.105;
RA   Yasuda H., Kamijo M., Honda R., Nakamura M., Hanaoka F., Ohba Y.;
RT   "A point mutation in C-terminal region of cdc2 kinase causes a
RT   G2-phase arrest in a mouse temperature-sensitive FM3A cell mutant.";
RL   Cell Struct. Funct. 16:105-112(1991).
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RX   PubMed=2208288; DOI=10.1016/0092-8674(90)90164-a;
RA   Th'ng J.P.H., Wright P.S., Hamaguchi J., Lee M.G., Norbury C.J.,
RA   Nurse P., Bradbury E.M.;
RT   "The FT210 cell line is a mouse G2 phase mutant with a
RT   temperature-sensitive CDC2 gene product.";
RL   Cell 63:313-324(1990).
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RX   PubMed=2428648; DOI=10.1016/0014-4827(86)90203-x;
RA   Mineo C., Murakami Y., Ishimi Y., Hanaoka F., Yamada M.-a.;
RT   "Isolation and analysis of a mammalian temperature-sensitive mutant
RT   defective in G2 functions.";
RL   Exp. Cell Res. 167:53-62(1986).
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RX   PubMed=9043671; DOI=10.1016/S0968-0896(96)00207-6;
RA   Osada H., Cui C.-B., Onose R., Hanaoka F.;
RT   "Screening of cell cycle inhibitors from microbial metabolites by a
RT   bioassay using a mouse cdc2 mutant cell line, tsFT210.";
RL   Bioorg. Med. Chem. 5:193-203(1997).
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