Cellosaurus cell line HMC-1.3 clone 2E2 (CVCL

Cellosaurus HMC-1.3 clone 2E2 (CVCL_E8DW)

Cross-references
Cell line name	HMC-1.3 clone 2E2
Accession	CVCL_E8DW
Resource Identification Initiative	To cite this cell line use: HMC-1.3 clone 2E2 (RRID:CVCL_E8DW)
Comments	Characteristics: Compared to the parent cell line has reduced activity in pathways involved in survival, cell-to-cell adhesion, and neoplasia with differences in expression of molecular components and cell surface markers (PubMed=37025992). Characteristics: Produces significantly smaller tumors than the parent cell line when subcutaneously inoculated in mice (PubMed=37025992). Doubling time: 36-48 hours (ABM=T8389). Omics: Transcriptomics; RNAseq. Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178. Cell type: Mast cell; CL=CL_0000097.
Sequence variations	Mutation; HGNC; HGNC:6342; KIT; Simple_corrected; p.Val560Gly (c.1679T>G); ClinVar=VCV000375916; Zygosity=Heterozygous; Note=By CRISPR/Cas9 (PubMed=37025992). Mutation; HGNC; HGNC:6342; KIT; Simple; p.Asp816Val (c.2447A>T); ClinVar=VCV000013852; Zygosity=Heterozygous (PubMed=37025992).
Disease	Mast cell leukemia (NCIt: C3169) Mast cell leukemia (ORDO: Orphanet_98851)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_H205 (HMC-1.2)
Sex of cell	Male
Age at sampling	52Y
Category	Cancer cell line
Publications	PubMed=37025992; DOI=10.3389/fimmu.2023.1078958; PMCID=PMC10071028 Bandara G., Falduto G.H., Luker A.J., Bai Y., Pfeiffer A., Lack J.B., Metcalfe D.D., Olivera A. CRISPR/Cas9-engineering of HMC-1.2 cells renders a human mast cell line with a single D816V-KIT mutation: an improved preclinical model for research on mastocytosis. Front. Immunol. 14:1078958.1-1078958.17(2023)
Cell line collections (Providers)	ABM; T8389
Gene expression databases	GEO; GSM6674140 GEO; GSM6674141 GEO; GSM6674142
Entry history
Entry creation	10-Apr-2025
Last entry update	10-Apr-2025
Version number	1