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Cellosaurus HEK-293-F TIMP-2-EK-6XHis (CVCL_E8D9)

[Text version]
Cell line name HEK-293-F TIMP-2-EK-6XHis
Accession CVCL_E8D9
Resource Identification Initiative To cite this cell line use: HEK-293-F TIMP-2-EK-6XHis (RRID:CVCL_E8D9)
Comments Characteristics: Transfected with a construct that contains an enterokinase cleavage site (EK) inserted between the C-terminus of TIMP2 and a six-histidine tag (6XHis) (PubMed=29140689).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11821; TIMP2.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D603 (FreeStyle 293-F)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=29140689; DOI=10.1021/acs.biochem.7b00700; PMCID=PMC6322544
Chowdhury A., Brinson R.G., Wei B.-Y., Stetler-Stevenson W.G.
Tissue inhibitor of metalloprotease-2 (TIMP-2): bioprocess development, physicochemical, biochemical, and biological characterization of highly expressed recombinant protein.
Biochemistry 56:6423-6433(2017)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1