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Cellosaurus CHS-20 (CVCL_E8BL)

[Text version]
Cell line name CHS-20
Accession CVCL_E8BL
Resource Identification Initiative To cite this cell line use: CHS-20 (RRID:CVCL_E8BL)
Comments Group: Non-human primate cell line.
Characteristics: Transfected with a pFRT/lacZeo construct that contains a FRT site for subsequent targeted integration of a Flp-In expression vector (from parent cell line).
Virology: Susceptible to infection by peste des petits ruminants virus (PPRV). Infection of this cell by PPRV resulted in the development of syncytia (PubMed=21371505).
Genetic integration: Method=Transfection; Gene=UniProtKB; Q283L5; Capra hircus SLAMF1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) (NCBI Taxonomy: 9534)
Hierarchy Parent: CVCL_U425 (Flp-In-CV-1)
Sex of cell Male
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=21371505; DOI=10.1016/j.jviromet.2011.02.024; PMCID=PMC3166437
Adombi C.M., Lelenta M., Lamien C.E., Shamaki D., Koffi Y.M., Traore A., Silber R., Couacy-Hymann E., Bodjo S.C., Djaman J.A., Luckins A.G., Diallo A.
Monkey CV1 cell line expressing the sheep-goat SLAM protein: a highly sensitive cell line for the isolation of peste des petits ruminants virus from pathological specimens.
J. Virol. Methods 173:306-313(2011)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1