Cellosaurus KTRC SIX2:CreERT2/GAPDH:dual (CVCL_E6IK)
| Cell line name | KTRC SIX2:CreERT2/GAPDH:dual |
|---|---|
| Synonyms | Kidney Translational Research Center SIX2:CreERT2/GAPDH:dual iPSC |
| Accession | CVCL_E6IK |
| Resource Identification Initiative | To cite this cell line use: KTRC SIX2:CreERT2/GAPDH:dual (RRID:CVCL_E6IK) |
| Comments | Population: Caucasian. Characteristics: Endogenous SIX2 activation combined with tamoxifen results in the activation of the Cre recombinase that activate the removal of EGFP and results in permanent switching of expression to mCherry (PubMed=31492807). Characteristics: Using CRISPR/Cas9 a T2A-CreERT2 construct has been inserted at the C-terminal of both alleles of SIX2 mCherry and a T2A-loxP-EGFP-loxP-mCherry construct has been inserted at the C-terminal of one allele of GAPDH (PubMed=31492807). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=CRISPR/Cas9; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2). Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471. Cell type: Fibroblast of foreskin; CL=CL_1001608. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_E5YY (KTRC SIX2:CreERT2) |
| Sex of cell | Male |
| Age at sampling | <1M |
| Category | Induced pluripotent stem cell |
| Web pages | Provider; KTRC; -; https://www.atlas-d2k.org/chaise/record/#2/Cell_Line:Reporter_Cell_Line/RID=16-DYTA |
| Publications | PubMed=31492807; DOI=10.1681/ASN.2019030303; PMCID=PMC6779359 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 10-Apr-2025 |
| Version number | 1 |