ID   KTRC SIX2:Cre/GAPDH:dual
AC   CVCL_E6IJ
SY   Kidney Translational Research Center SIX2:Cre/GAPDH:dual iPSC
RX   PubMed=31492807;
WW   Provider; KTRC; -; https://www.atlas-d2k.org/chaise/record/#2/Cell_Line:Reporter_Cell_Line/RID=16-1YZT
CC   Population: Caucasian.
CC   Characteristics: Endogenous SIX2 activation results in the activation of the Cre recombinase that activate the removal of EGFP and results in permanent switching of expression to mCherry (PubMed=31492807).
CC   Characteristics: Using CRISPR/Cas9 a T2A-Cre construct has been inserted at the C-terminal of both alleles of SIX2 mCherry and a T2A-loxP-EGFP-loxP-mCherry construct has been inserted at the C-terminal of one allele of GAPDH (PubMed=31492807).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P06956; Escherichia phage P1 cre.
CC   Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
CC   Cell type: Fibroblast of foreskin; CL=CL_1001608.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_E5YX ! KTRC SIX2:Cre
SX   Male
AG   <1M
CA   Induced pluripotent stem cell
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=31492807; DOI=10.1681/ASN.2019030303; PMCID=PMC6779359;
RA   Vanslambrouck J.M., Wilson S.B., Tan K.S., Soo J.Y.-C., Scurr M.,
RA   Spijker H.S., Starks L.T., Neilson A., Cui X.-X., Jain S., Little M.H.,
RA   Howden S.E.;
RT   "A toolbox to characterize human induced pluripotent stem cell-derived
RT   kidney cell types and organoids.";
RL   J. Am. Soc. Nephrol. 30:1811-1823(2019).
//