Cellosaurus FIRE2FLIPCS clone 1 (CVCL_E6HT)
| Cell line name | FIRE2FLIPCS clone 1 |
|---|---|
| Synonyms | FIRE2FLPCS-520-8 |
| Accession | CVCL_E6HT |
| Resource Identification Initiative | To cite this cell line use: FIRE2FLIPCS clone 1 (RRID:CVCL_E6HT) |
| Comments | Characteristics: Expresses a Fe-IRE (FIRE) biosensor that senses IRP activity and cellular iron status (PubMed=39824193). Characteristics: This cell line contains a FIRE2 biosensor (emiRFP670-sensor, mRuby3-control) and is transposed with two PiggyBac constructs FIRES-FLIP-PBCS-E670 and FIREC-PBCS-MR3 flanking FIRE sensor and control fluorescent protein, respectively. The FLIP-FIRE sensor is composed of a 740 bp sequence synthesized in backwards orientation and based on known mammalian IRP1/2 binding sites (PubMed=39824193). Genetic integration: Method=PiggyBac transposition; Gene=FPbase; YHJV5; emiRFP670 (Note=Constitutively fluorescent near IR fluoresent protein from R.palustris). Genetic integration: Method=PiggyBac transposition; Gene=FPbase; MZMFB; mRuby3 (Note=Bright red fluorescent protein derived from eqFP611). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6J x 129S4/SvJae. |
| Hierarchy | Parent: CVCL_C865 (v6.5) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=39824193; DOI=10.1016/j.crmeth.2024.100960 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 10-Apr-2025 |
| Version number | 1 |