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Cellosaurus KTRC MAFB:mTagBFP2/GATA3:mCherry (CVCL_E5VU)

[Text version]
Cell line name KTRC MAFB:mTagBFP2/GATA3:mCherry
Synonyms Kidney Translational Research Center MAFB:mTagBFP2/GATA3:mCherry iPSC
Accession CVCL_E5VU
Resource Identification Initiative To cite this cell line use: KTRC MAFB:mTagBFP2/GATA3:mCherry (RRID:CVCL_E5VU)
Comments Population: Caucasian.
Characteristics: Identifies podocytes (MAFB+), collecting duct/connecting segment (GATA3+), GATA3+ interstitial population (PubMed=31492807).
Characteristics: Using CRISPR/Cas9 MAFB has been endogenously tagged at the N-terminal of one allele with mTagBFP2 and GATA3 has been endogenously tagged at the C-terminal in one allele with mCherry (PubMed=31492807).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZO7NN; mTagBFP2 (Note=Blue fluorescent protein derived from eqFP578).
Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
Cell type: Fibroblast of foreskin; CL=CL_1001608.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E5VS (KTRC MAFB:mTagBFP2)
Sex of cell Male
Age at sampling <1M
Category Induced pluripotent stem cell
Web pages Provider; KTRC; -; https://www.atlas-d2k.org/chaise/record/#2/Cell_Line:Reporter_Cell_Line/RID=14-3QDG
Publications

PubMed=31492807; DOI=10.1681/ASN.2019030303; PMCID=PMC6779359
Vanslambrouck J.M., Wilson S.B., Tan K.S., Soo J.Y.-C., Scurr M., Spijker H.S., Starks L.T., Neilson A., Cui X.-X., Jain S., Little M.H., Howden S.E.
A toolbox to characterize human induced pluripotent stem cell-derived kidney cell types and organoids.
J. Am. Soc. Nephrol. 30:1811-1823(2019)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1