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Cellosaurus CHO NB1-5 (CVCL_E4I7)

[Text version]
Cell line name CHO NB1-5
Synonyms NB1-5; NB
Accession CVCL_E4I7
Resource Identification Initiative To cite this cell line use: CHO NB1-5 (RRID:CVCL_E4I7)
Comments Genetic integration: Method=Transfection; Gene=MGI; MGI:95824; Nr3c1 (Note=With p.Arg493His).
Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_1977 (CHO-DXB11)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=2157974; DOI=10.1210/mend-4-1-162
Hirst M.A., Northrop J.P., Danielsen M., Ringold G.M.
High level expression of wild type and variant mouse glucocorticoid receptors in Chinese hamster ovary cells.
Mol. Endocrinol. 4:162-170(1990)

PubMed=2243084; DOI=10.1016/S0021-9258(17)30478-7
Sanchez E.R., Hirst M.A., Scherrer L.C., Tang H.-Y., Welsh M.J., Harmon J.M., Simons S.S. Jr., Ringold G.M., Pratt W.B.
Hormone-free mouse glucocorticoid receptors overexpressed in Chinese hamster ovary cells are localized to the nucleus and are associated with both hsp70 and hsp90.
J. Biol. Chem. 265:20123-20130(1990)

Entry history
Entry creation19-Dec-2024
Last entry update19-Dec-2024
Version number1