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Cellosaurus CHO SSF3KVN1 (CVCL_E4HU)

[Text version]
Cell line name CHO SSF3KVN1
Synonyms SSF3KVN1
Accession CVCL_E4HU
Resource Identification Initiative To cite this cell line use: CHO SSF3KVN1 (RRID:CVCL_E4HU)
Comments Group: Serum/protein free medium cell line.
Characteristics: The expression of VTN is under transcriptional control of a DEX-inducible MMTV promoter. Displays constitutively adherent growth (PubMed=10516577).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:7978; NR3C1.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:12724; VTN.
Genetic integration: Method=Transfection; Gene=UniProtKB; P0A9M5; Escherichia coli gpt.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_E4HT (CHO SSF3GR)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=10516577; DOI=10.1002/(SICI)1097-0290(19991205)65:5<523::AID-BIT4>3.0.CO;2-6
Gandor C.R., Zang-Gandor M.O., Flor P.J., Marangi M., Asselbergs F.A.M.
Conditionally adherent growth of serum-independent CHO cells for automated drug screening and biopharmaceutical production.
Biotechnol. Bioeng. 65:523-528(1999)

Entry history
Entry creation19-Dec-2024
Last entry update19-Dec-2024
Version number1