ID   CHO E-10C6
AC   CVCL_E4HQ
SY   E-10C6
RX   Patent=EP0088540A2;
RX   Patent=US5004689;
RX   PubMed=6308636;
RX   PubMed=14705000;
RX   PubMed=16003777;
CC   Group: Patented cell line.
CC   Sequence variation: Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:5438; IFNG.
CC   Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:94890; Dhfr.
CC   Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Transformant: NCIt; C44386; Gamma radiation.
CC   Caution: It is uncertain whether the cell line used in PubMed=14705000 and PubMed=16003777 is CHO E-10C6 or another cell line derived from CHO EC-10B (Cellosaurus=CVCL_E4HM) or CHO EC-10C (Cellosaurus=CVCL_E4HN).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_E4HN ! CHO E-10C
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 19-12-24; Last updated: 19-12-24; Version: 1
//
RX   Patent=EP0088540A2;
RA   Fiers W.C.;
RT   "DNA sequences, recombinant DNA molecules and processes for producing
RT   human immune interferon-like polypeptides.";
RL   Patent number EP0088540A2, 14-Sep-1983.
//
RX   Patent=US5004689;
RA   Fiers W.C., Allet B.;
RT   "DNA sequences, recombinant DNA molecules and processes for producing
RT   human gamma interferon-like polypeptides in high yields.";
RL   Patent number US5004689, 02-Apr-1991.
//
RX   PubMed=6308636; DOI=10.1073/pnas.80.15.4654; PMCID=PMC384102;
RA   Scahill S.J., Devos R., Van der Heyden J., Fiers W.C.;
RT   "Expression and characterization of the product of a human immune
RT   interferon cDNA gene in Chinese hamster ovary cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:4654-4658(1983).
//
RX   PubMed=14705000; DOI=10.1002/bit.10861;
RA   Fox S.R., Patel U.A., Yap M.G.-S., Wang D.I.-C.;
RT   "Maximizing interferon-gamma production by Chinese hamster ovary cells
RT   through temperature shift optimization: experimental and modeling.";
RL   Biotechnol. Bioeng. 85:177-184(2004).
//
RX   PubMed=16003777; DOI=10.1002/bit.20511;
RA   Wlaschin K.F., Nissom P.M., de Leon Gatti M., Ong P.-F., Arleen S.,
RA   Tan K.-S., Rink A., Cham B., Wong K.T.-K., Yap M.G.-S., Hu W.-S.;
RT   "EST sequencing for gene discovery in Chinese hamster ovary cells.";
RL   Biotechnol. Bioeng. 91:592-606(2005).
//