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Cellosaurus CHO SSF3 (CVCL_E4HJ)

[Text version]
Cell line name CHO SSF3
Synonyms CHOSSF3; SSF3
Accession CVCL_E4HJ
Resource Identification Initiative To cite this cell line use: CHO SSF3 (RRID:CVCL_E4HJ)
Comments Group: Serum/protein free medium cell line.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_1977 (CHO-DXB11)
Children:
CVCL_E4HK (CHO SSF3/pMOZ uPA clone 1)CVCL_E4HL (CHO SSF3/pMOZ uPA clone 8)CVCL_E4HT (CHO SSF3GR)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

DOI=10.3929/ethz-a-000888370
Gandor C.R.
Establishment and characterization of growth-factor-prototrophic Chinese hamster ovary (CHO) cell lines for the production of recombinant proteins.
Thesis PhD (1993); Swiss Federal Institute of Technology Zurich; Zurich; Switzerland

PubMed=8549740; DOI=10.1016/0014-5793(95)01328-8
Gandor C.R., Leist C.H., Fiechter A., Asselbergs F.A.M.
Amplification and expression of recombinant genes in serum-independent Chinese hamster ovary cells.
FEBS Lett. 377:290-294(1995)

PubMed=9634782; DOI=10.1038/nbt0495-389
Zang-Gandor M.O., Trautmann H., Gandor C.R., Messi F., Asselbergs F.A.M., Leist C.H., Fiechter A., Reiser J.
Production of recombinant proteins in Chinese hamster ovary cells using a protein-free cell culture medium.
Biotechnology (N.Y.) 13:389-392(1995)

PubMed=10516577; DOI=10.1002/(SICI)1097-0290(19991205)65:5<523::AID-BIT4>3.0.CO;2-6
Gandor C.R., Zang-Gandor M.O., Flor P.J., Marangi M., Asselbergs F.A.M.
Conditionally adherent growth of serum-independent CHO cells for automated drug screening and biopharmaceutical production.
Biotechnol. Bioeng. 65:523-528(1999)

Entry history
Entry creation19-Dec-2024
Last entry update19-Dec-2024
Version number1