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Cellosaurus UCSFi001-A-78 (CVCL_E4G4)

[Text version]
Cell line name UCSFi001-A-78
Synonyms WTC11-KL-GFP; CMCi014-A-78
Accession CVCL_E4G4
Resource Identification Initiative To cite this cell line use: UCSFi001-A-78 (RRID:CVCL_E4G4)
Comments From: The Catholic University of Korea; Seoul; South Korea.
Population: Japanese.
Characteristics: Using CRISPR-Cas9 a P2A-GFP-IRES-HygR construct was integrated at the 3'UTR region of KLOTHO (PubMed=39111000).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Publications

PubMed=39111000; DOI=10.1016/j.scr.2024.103499
Lim S.W., Lee K.I., Cui S., Fang X.-Y., Shin Y.J., Lee H., Lee J.Y., Chung B.H., Yang C.W.
Generation of green fluorescent protein reporter knock-in iPSC line at the 3'UTR region of the KLOTHO locus.
Stem Cell Res. 80:103499-103499(2024)

Cross-references
Cell line databases/resources hPSCreg; UCSFi001-A-78
Biological sample resources BioSamples; SAMEA115128015
Entry history
Entry creation19-Dec-2024
Last entry update19-Dec-2024
Version number1