ID   Nalm6-Fluc-Puro/HuCD19-Puro (mid)
AC   CVCL_E4BU
DR   Imanis; CL183
CC   Population: Caucasian.
CC   Characteristics: Reporter cell line, transduced with the LV-Fluc-P2A-PuroR vector encoding for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (from parent cell line).
CC   Characteristics: Transduced with a human CD19 and PuroR encoding lentivirus. A moderate CD19 expressing population was generated by selection using a methylcellulose based semi-solid medium (Imanis=CL183).
CC   Sequence variation: Gene fusion; HGNC; HGNC:50800; DUX4 + HGNC; HGNC:5477; IGH; Name(s)=IGH-DUX4; Note=The translocation occurs in the silenced IGH allele (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:3236; EGFR; Simple; p.Arg1068Ter (c.3202C>T); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Ala146Thr (c.436G>A); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:9864; RARA; Simple; p.Gln45Ter (c.133C>T); ClinVar=VCV000393019; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1633; CD19.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
DI   NCIt; C9143; Adult B acute lymphoblastic leukemia
DI   ORDO; Orphanet_99860; Precursor B-cell acute lymphoblastic leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_RA36 ! Nalm6-Fluc-Puro
SX   Male
AG   19Y
CA   Cancer cell line
DT   Created: 19-12-24; Last updated: 19-12-24; Version: 1
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