Cellosaurus cell line Jurkat-Lucia TCR-hPD-1 (CVCL

Cellosaurus Jurkat-Lucia TCR-hPD-1 (CVCL_E3JE)

Entry history
Cell line name	Jurkat-Lucia TCR-hPD-1
Accession	CVCL_E3JE
Resource Identification Initiative	To cite this cell line use: Jurkat-Lucia TCR-hPD-1 (RRID:CVCL_E3JE)
Comments	Population: Caucasian. Biotechnology: Used in the Jurkat-Raji PD-1/PD-L1 assay (Bio-IC) specifically designed for screening antibody-, Fc-fusion protein-, or small-molecule antagonists of the PD-1/PD-L1 immune checkpoint (IC) axis (Invivogen). Characteristics: Express a specific TCR and PDCD1 (PD-1) along with the Lucia luciferase reporter gene under the control of an ISG54 minimal promoter fused to six NFAT response elements (InvivoGen). Genetic integration: Method=Transfection; Gene=HGNC; HGNC:8760; PDCD1. Genetic integration: Method=Transfection; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178. Cell type: T-cell; CL=CL_0000084.
Sequence variations	Mutation; HGNC; HGNC:959; BAX; Simple; p.Glu41Argfs19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:959; BAX; Simple; p.Glu41Glyfs33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
Disease	Childhood T acute lymphoblastic leukemia (NCIt: C7953) Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_KS47 (Jurkat-Lucia NFAT)
Sex of cell	Male
Age at sampling	14Y
Category	Cancer cell line
Web pages	https://www.invivogen.com/hpd1-bioassay
Entry creation	19-Dec-2024
Last entry update	19-Dec-2024
Version number	1