ID   CHO TSHR-10,000
AC   CVCL_E3EM
SY   TSHR-10,0000
RX   PubMed=8895321;
CC   Characteristics: Adapted to growth in 10000 nM methotrexate (PubMed=8895321).
CC   Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
CC   Sequence variation: Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:12373; TSHR (Note=With deleted untranslated regions).
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
CC   Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_E3EL ! CHO TSHR-800
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 19-12-24; Last updated: 19-12-24; Version: 1
//
RX   PubMed=8895321; DOI=10.1210/endo.137.11.8895321;
RA   Chazenbalk G.D., Kakinuma A., Jaume J.C., McLachlan S.M., Rapoport B.;
RT   "Evidence for negative cooperativity among human thyrotropin receptors
RT   overexpressed in mammalian cells.";
RL   Endocrinology 137:4586-4591(1996).
//