ID   CHO-TPO-M1-K1
AC   CVCL_E3EG
RX   PubMed=1936519;
RX   PubMed=2274058;
CC   Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
CC   Sequence variation: Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:12015; TPO (Note=With p.Trp849_Leu933del, secreted = TPO-M1).
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
CC   Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_7180 ! CHO-DG44
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 19-12-24; Last updated: 19-12-24; Version: 1
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RX   PubMed=1936519; DOI=10.1016/0303-7207(91)90191-t;
RA   Kaufman K.D., Foti D.P., Seto P., Rapoport B.;
RT   "Overexpression of an immunologically-intact, secreted form of human
RT   thyroid peroxidase in eukaryotic cells.";
RL   Mol. Cell. Endocrinol. 78:107-114(1991).
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RX   PubMed=2274058; DOI=10.1210/mend-4-5-786;
RA   Foti D.P., Kaufman K.D., Chazenbalk G.D., Rapoport B.;
RT   "Generation of a biologically active, secreted form of human thyroid
RT   peroxidase by site-directed mutagenesis.";
RL   Mol. Endocrinol. 4:786-791(1990).
//