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Cellosaurus PT54 (CVCL_E3E0)

[Text version]
Cell line name PT54
Accession CVCL_E3E0
Resource Identification Initiative To cite this cell line use: PT54 (RRID:CVCL_E3E0)
Comments Characteristics: Defective in Pex2 (PubMed=10771091).
Characteristics: Selected using the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method. P9OH acts as a photosensitizer. Normal cells which can incorporate the fluorescent fatty alcohol into lipids are killed by UV, but mutants who incorporate P9OH poorly are less susceptible to photosensitized killing.
Characteristics: Transfected with a GFP N-terminally tagged with peroxisomal PTS2 signal (PubMed=10771091).
Genetic integration: Method=Transfection; Gene=FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_E3DW (CHOPS2a)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=10771091; DOI=10.1016/S0167-4889(00)00019-7
Ito M., Ito R., Huang Y., Miura S., Imamura A., Suzuki Y., Shimozawa N.
Rapid isolation and characterization of CHO mutants deficient in peroxisome biogenesis using the peroxisomal forms of fluorescent proteins.
Biochim. Biophys. Acta 1496:232-242(2000)

PubMed=11330054; DOI=10.1385/CBB:32:1-3:253
Ito M., Ito R., Miura S., Huang Y.
Isolation of peroxisome-defective CHO mutant cells using green fluorescent protein.
Cell Biochem. Biophys. 32:253-257(2000)

Entry history
Entry creation10-Sep-2024
Last entry update10-Sep-2024
Version number1