ID   PT13
AC   CVCL_E3DY
RX   PubMed=10771091;
RX   PubMed=11330054;
CC   Characteristics: Selected using the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method. P9OH acts as a photosensitizer. Normal cells which can incorporate the fluorescent fatty alcohol into lipids are killed by UV, but mutants who incorporate P9OH poorly are less susceptible to photosensitized killing.
CC   Characteristics: Transfected with a GFP N-terminally tagged with peroxisomal PTS2 signal (PubMed=10771091).
CC   Genetic integration: Method=Transfection; Gene=FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_E3DW ! CHOPS2a
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 10-09-24; Last updated: 10-09-24; Version: 1
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RX   PubMed=10771091; DOI=10.1016/S0167-4889(00)00019-7;
RA   Ito M., Ito R., Huang Y., Miura S., Imamura A., Suzuki Y.,
RA   Shimozawa N.;
RT   "Rapid isolation and characterization of CHO mutants deficient in
RT   peroxisome biogenesis using the peroxisomal forms of fluorescent
RT   proteins.";
RL   Biochim. Biophys. Acta 1496:232-242(2000).
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RX   PubMed=11330054; DOI=10.1385/CBB:32:1-3:253;
RA   Ito M., Ito R., Miura S., Huang Y.;
RT   "Isolation of peroxisome-defective CHO mutant cells using green
RT   fluorescent protein.";
RL   Cell Biochem. Biophys. 32:253-257(2000).
//