ID   HEK293 TL4-2B
AC   CVCL_E3BY
SY   TL4-2B
RX   PubMed=35885927;
CC   Characteristics: Using pcDNA5/FRT/TO and pOG44, a linear RNA encompassing exons 2B, 3 and 4 of human SMN gene was integrated in a single site and its expression is regulated by doxycycline (PubMed=35885927).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; 11117; SMN1 (Note=Exons 2B, 3 and 4).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D585 ! T-REx-293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 10-09-24; Last updated: 10-09-24; Version: 1
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RX   PubMed=35885927; DOI=10.3390/genes13071145; PMCID=PMC9323214;
RA   Luo D., Singh N.N., Singh R.N.;
RT   "Internal introns promote backsplicing to generate circular RNAs from
RT   spinal muscular atrophy gene.";
RL   Genes (Basel) 13:1145.1-1145.20(2022).
//