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Cellosaurus HEK293T ADRM1 K21R/K34R agDD-GFP (CVCL_E3BM)

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Cell line name HEK293T ADRM1 K21R/K34R agDD-GFP
Synonyms HEK293T::ADRM1K21RK34R expressing agDD-GFP
Accession CVCL_E3BM
Resource Identification Initiative To cite this cell line use: HEK293T ADRM1 K21R/K34R agDD-GFP (RRID:CVCL_E3BM)
Comments Characteristics: Stably expresses agDD-GFP which consists of an aggregating destabilizing domain (AgDD) fused to the N-terminal of Superfolder GFP.
Genetic integration: Method=Transduction; Gene=FPbase; B4SOW; Superfolder GFP (Note=sfGFP, superfolding variant of GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Miscellaneous: Cell line information from personal communication of Harper, Jeffrey Wade.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Sequence variations
  • Mutation; HGNC; HGNC:15759; ADRM1; Simple_edited; p.Lys21Arg (c.116A>G); Zygosity=Homozygous; Note=By CRISPR/Cas9 (Direct_author_submission).
  • Mutation; HGNC; HGNC:15759; ADRM1; Simple_edited; p.Lys34Arg (c.155A>G); Zygosity=Homozygous; Note=By CRISPR/Cas9 (Direct_author_submission).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E3BL (HEK293T ADRM1 K21R/K34R)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Entry history
Entry creation10-Sep-2024
Last entry update19-Dec-2024
Version number2