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Cellosaurus HEK293T UBE3C-/- agDD-GFP (CVCL_E3BK)

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Cell line name HEK293T UBE3C-/- agDD-GFP
Synonyms HEK293T::UBE3C-/- expressing agDD-GFP
Accession CVCL_E3BK
Resource Identification Initiative To cite this cell line use: HEK293T UBE3C-/- agDD-GFP (RRID:CVCL_E3BK)
Comments Characteristics: Stably expresses agDD-GFP which consists of an aggregating destabilizing domain (AgDD) fused to the N-terminal of Superfolder GFP.
Knockout cell: Method=CRISPR/Cas9; HGNC; 16803; UBE3C.
Genetic integration: Method=Transduction; Gene=FPbase; B4SOW; Superfolder GFP (Note=sfGFP, superfolding variant of GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Miscellaneous: Cell line information from personal communication of Harper J.W.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E3BJ (HEK293T UBE3C-/-)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Entry history
Entry creation10-Sep-2024
Last entry update10-Sep-2024
Version number1