Cellosaurus HEK293T UBE3C-/- agDD-GFP (CVCL_E3BK)
| Cell line name | HEK293T UBE3C-/- agDD-GFP |
|---|---|
| Synonyms | HEK293T::UBE3C-/- expressing agDD-GFP |
| Accession | CVCL_E3BK |
| Resource Identification Initiative | To cite this cell line use: HEK293T UBE3C-/- agDD-GFP (RRID:CVCL_E3BK) |
| Comments | Characteristics: Stably expresses agDD-GFP which consists of an aggregating destabilizing domain (AgDD) fused to the N-terminal of Superfolder GFP. Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:16803; UBE3C. Genetic integration: Method=Transduction; Gene=FPbase; B4SOW; Superfolder GFP (Note=sfGFP, superfolding variant of GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA). Miscellaneous: Cell line information from personal communication of Harper, Jeffrey Wade. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_E3BJ (HEK293T UBE3C-/-) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Entry history | |
| Entry creation | 10-Sep-2024 |
| Last entry update | 19-Dec-2024 |
| Version number | 2 |