ID   CHSE-EC-PKR-C28
AC   CVCL_E3AF
SY   EC-PKR-C28
RX   PubMed=39058317;
CC   Group: Fish cell line.
CC   Characteristics: The parent cell line was transiently transfected with a mix of sgRNAs designed against eif2ak2/pkr and against mEGFP. mEGFP-deficient cells were isolated. As this particular clone is not KO for eif2ak2 it is used as a control cell line (PubMed=39058317).
CC   Sequence variation: Mutation; UniProtKB; A0A8C7HVT8; eif2ak2; Simple_edited; p.Cys11_Glu12del (c.30_35delGTGCGA); Zygosity=Homozygous; Note=By CRISPR/Cas9 (PubMed=39058317).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=Nuclear version; nCas9n).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Epithelial cell; CL=CL_0000066.
OX   NCBI_TaxID=74940; ! Oncorhynchus tshawytscha (Chinook salmon)
HI   CVCL_DG46 ! CHSE-EC
SX   Sex unspecified
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 10-09-24; Last updated: 10-09-24; Version: 1
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RX   PubMed=39058317; DOI=10.4049/jimmunol.2400076;
RA   Chaumont L., Peruzzi M., Huetz F., Raffy C., Le Hir J., Minke J.,
RA   Boudinot P., Collet B.;
RT   "Salmonid double-stranded RNA-dependent protein kinase activates
RT   apoptosis and inhibits protein synthesis.";
RL   J. Immunol. 213:700-717(2024).
//