ID   H9 AAVS1-TRE3G-NGN2 3xFLAG-EEA1 TMEM230-/-
AC   CVCL_E2VV
SY   H9 ES AAVS1-NGN2 Flag-EEA1 TMEM230-/-
CC   Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line).
CC   Characteristics: Using CRISPR/Cas9 EAA1 was N-terminally tagged on both alleles with a 3xFLAG tag (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:15876; TMEM230.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2.
CC   Miscellaneous: Cell line information from personal communication of Harper, Jeffrey Wade.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D1KV ! H9 AAVS1-TRE3G-NGN2 3xFLAG-EEA1
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 10-09-24; Last updated: 19-12-24; Version: 2
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