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Cellosaurus HEK293T SLC35A1 KO (CVCL_E2QG)

[Text version]
Cell line name HEK293T SLC35A1 KO
Synonyms HEK293T-SLC35A1-KO
Accession CVCL_E2QG
Resource Identification Initiative To cite this cell line use: HEK293T SLC35A1 KO (RRID:CVCL_E2QG)
Comments Doubling time: ~24 hours (DSMZ=ACC-988).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:11021; SLC35A1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
STR profile Source(s): DSMZ=ACC-988

Markers:
AmelogeninX
CSF1PO11,12
D2S133818,19
D3S135816,17
D5S8188,9
D7S82010,11,12
D8S117911,12,13,14
D13S31712,13
D16S5399,12,13
D18S5116,17,18
D19S43316,17,18
D21S1128,29,30
FGA21,22,23
Penta D9,10
Penta E7,15,16
TH019.3
TPOX11
vWA16,20

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Publications

PubMed=34069698; DOI=10.3390/cells10051259; PMCID=PMC8160606
Banning A., Zakrzewicz A., Chen X., Gray S.J., Tikkanen R.
Knockout of the CMP-sialic acid transporter SLC35A1 in human cell lines increases transduction efficiency of adeno-associated virus 9: implications for gene therapy potency assays.
Cells 10:1259.1-1259.14(2021)

Cross-references
Cell line collections (Providers) DSMZ; ACC-988
Entry history
Entry creation10-Sep-2024
Last entry update19-Dec-2024
Version number2