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Cellosaurus HCT-116 MTBP-mClover-XLoneGemininT25D (CVCL_E0W0)

[Text version]
Cell line name HCT-116 MTBP-mClover-XLoneGemininT25D
Accession CVCL_E0W0
Resource Identification Initiative To cite this cell line use: HCT-116 MTBP-mClover-XLoneGemininT25D (RRID:CVCL_E0W0)
Comments Population: Caucasian.
Characteristics: Transfected with two plasmids, XLone-GemininT25D and pCMV-hyPBase. The XLone-GemininT25D plasmid contains in one orientation a EF1A-rtTA-P2A-Bsd region where rtTA is a reverse tetracycline-controlled transactivator, a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 and in the other orientation a TRE3G-GemininT25D region thus allowing the doxycycline induction of gemimin (DOI=10.1101/2024.02.02.578516).
Characteristics: Using CRISPR/Cas9 the fluorescent protein mClover was integrated at the C-terminus of MTBP (from parent cell line).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 4Z641; Clover (Note=Basic green/yellow fluorescent protein derived from GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Thr25Asp; stabilized against degradation).
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Leu450Ter (c.1349delT) (p.Leu450fs) (c.1344delT); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C7SR (HCT-116 MTBP-mClover)
Sex of cell Male
Age at sampling 48Y
Category Cancer cell line
Publications

DOI=10.1101/2024.02.02.578516
Noble T.D., Sansam C.G., Wittig K.A., Majchrzycka B., Sansam C.L.
Cell cycle-dependent TICRR/TRESLIN and MTBP chromatin binding mechanisms and patterns.
bioRxiv 2024:02.02.578516-02.02.578516(2024)

Entry history
Entry creation10-Sep-2024
Last entry update19-Dec-2024
Version number2