ID   NMuMG/Fucci
AC   CVCL_E069
SY   NMuMG/Fucci #1; NMuMG-Fucci
DR   CLO; CLO_0050967
DR   RCB; RCB2813
DR   Wikidata; Q54930850
RX   PubMed=18267078;
CC   Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
CC   Characteristics: Transfected with the Fucci probe which consist of the fusion of monomeric Kusabira-Orange2 (mKO2) to the ubiquitination domain of human CDT1 (AA 30-120) and monomeric AzamiGreen (mAG) to the ubiquitination domain of human geminin (AA 1-110).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; HC91W; AzamiGreen (Note=Green fluorescent protein from G.fascicularis).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; 134NN; mKusabira-Orange2 (Note=mKO2, orange fluorescent protein derived from F.concinna Kusabira-Orange (KO)).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Met1_Thr29del and p.Ser121_Leu546del).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del).
CC   Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
CC   Breed/subspecies: Namru.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0075 ! NMuMG
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 22-10-12; Last updated: 10-04-25; Version: 13
//
RX   PubMed=18267078; DOI=10.1016/j.cell.2007.12.033;
RA   Sakaue-Sawano, Asako
RA   Kurokawa, Hiroshi
RA   Morimura, Toshifumi
RA   Hanyu, Aki
RA   Hama, Hiroshi
RA   Osawa, Hatsuki
RA   Kashiwagi, Saori
RA   Fukami, Kiyoko
RA   Miyata, Takaki
RA   Miyoshi, Hiroyuki
RA   Imamura, Takeshi
RA   Ogawa, Masaharu
RA   Masai, Hisao
RA   Miyawaki, Atsushi
RT   "Visualizing spatiotemporal dynamics of multicellular cell-cycle
RT   progression.";
RL   Cell 132:487-498(2008).
//