Cellosaurus cell line RKO Cas9 (CVCL

Cross-references
Cell line name	RKO Cas9
Accession	CVCL_DX82
Resource Identification Initiative	To cite this cell line use: RKO Cas9 (RRID:CVCL_DX82)
Comments	Characteristics: Transduced with a lentiviral vector EF1a-Bsd-T2A-Cas9-WPRE (Horizon_Discovery=HD+Cas9-007). Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Genetic integration: Method=Transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations	Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; 11773; TGFBR2; Simple; p.Leu452Pro (c.1355T>C); ClinVar=VCV000930524; Zygosity=Heterozygous (from parent cell line).
Disease	Colon carcinoma (NCIt: C4910)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0504 (RKO)
Sex of cell	Sex unspecified
Age at sampling	Age unspecified
Category	Cancer cell line
Cell line collections (Providers)	Horizon_Discovery; HD+Cas9-007
Encyclopedic resources	Wikidata; Q127384052
Entry history
Entry creation	30-Jan-2024
Last entry update	10-Sep-2024
Version number	2