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Cellosaurus HA-CHO clone 4B (CVCL_DD04)

[Text version]
Cell line name HA-CHO clone 4B
Synonyms 4B
Accession CVCL_DD04
Resource Identification Initiative To cite this cell line use: HA-CHO clone 4B (RRID:CVCL_DD04)
Comments Characteristics: Temperature defective (at 39 Celsius) expression of hemagglutinin (HA) on the cell surface (PubMed=2537314).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03451; Influenza A virus isolate A/Japan/305/1957 HA.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_DD03 (HA-CHO)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=2537314; DOI=10.1083/jcb.108.2.339; PMCID=PMC2115425
Hearing J., Hunter E., Rodgers L., Gething M.-J., Sambrook J.F.
Isolation of Chinese hamster ovary cell lines temperature conditional for the cell-surface expression of integral membrane glycoproteins.
J. Cell Biol. 108:339-353(1989)

PubMed=2267628; DOI=10.1007/BF01233094
Beck P.J., Gething M.-J., Sambrook J.F., Lehrman M.A.
Complementing mutant alleles define three loci involved in mannosylation of Man5-GlcNAc2-P-P-dolichol in Chinese hamster ovary cells.
Somat. Cell Mol. Genet. 16:539-548(1990)

Cross-references
Encyclopedic resources Wikidata; Q54872260
Entry history
Entry creation13-Jul-2016
Last entry update19-Dec-2024
Version number11