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Cellosaurus H1L6.1c3 (CVCL_DC23)

[Text version]
Cell line name H1L6.1c3
Accession CVCL_DC23
Resource Identification Initiative To cite this cell line use: H1L6.1c3 (RRID:CVCL_DC23)
Comments Biotechnology: Cell line developed for CALUX (Chemically Activated LUciferase eXpression) bioassay. CALUX is used for rapid screening of samples for the presence of dioxin/TCDD-like compounds and AhR agonists/antagonists.
Biotechnology: Used in US Environmental Protection Agency (EPA) hazardous waste test method 4435 (SW-846).
Characteristics: Acts through the activation of a luciferase reporter gene under the control of dioxin-response elements (DREs).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Firefly luciferase.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Disease Hepatocellular carcinoma of the mouse (NCIt: C38756)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57L/J.
Hierarchy Parent: CVCL_0328 (Hepa-1c1c7)
Sex of cell Female
Category Cancer cell line
Web pages Info; EPA; -; https://www.epa.gov/hw-sw846/sw-846-test-method-4435-screening-dioxin-chemical-activity-soils-and-sediments-using-calux
Provider; XDSI; -; http://www.dioxins.com/products/
Publications

PubMed=18969542; DOI=10.1016/j.talanta.2004.05.032
Denison M.S., Zhao B., Baston D.S., Clark G.C., Murata H., Han D.
Recombinant cell bioassay systems for the detection and relative quantitation of halogenated dioxins and related chemicals.
Talanta 63:1123-1133(2004)

PubMed=21872055; DOI=10.1016/j.talanta.2011.07.042
Van Langenhove K., Croes K., Denison M.S., Elskens M., Baeyens W.
The CALUX bio-assay: analytical comparison between mouse hepatoma cell lines with a low (H1L6.1c3) and high (H1L7.5c1) number of dioxin response elements.
Talanta 85:2039-2046(2011)

PubMed=26366531; DOI=10.1021/acs.est.5b02906; PMCID=PMC4772899
Brennan J.C., He G.-C., Tsutsumi T., Zhao J., Wirth E., Fulton M.H., Denison M.S.
Development of species-specific Ah receptor-responsive third generation CALUX cell lines with enhanced responsiveness and improved detection limits.
Environ. Sci. Technol. 49:11903-11912(2015)

Cross-references
Encyclopedic resources Wikidata; Q54871989
Entry history
Entry creation13-Jul-2016
Last entry update10-Apr-2025
Version number12