ID   L-WR
AC   CVCL_DA05
SY   L-Wnt3a/R-spondin
DR   Wikidata; Q54900913
RX   PubMed=24232249;
CC   Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:1920030; Rspo3.
CC   Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:98956; Wnt3a.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Connective tissue; UBERON=UBERON_0002384.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: C3H/An.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0635 ! L Wnt-3A
SX   Male
AG   100D
CA   Spontaneously immortalized cell line
DT   Created: 13-07-16; Last updated: 10-09-24; Version: 8
//
RX   PubMed=24232249; DOI=10.1038/nprot.2013.153; PMCID=PMC3969856;
RA   Miyoshi H., Stappenbeck T.S.;
RT   "In vitro expansion and genetic modification of gastrointestinal stem
RT   cells in spheroid culture.";
RL   Nat. Protoc. 8:2471-2482(2013).
//