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Cellosaurus HEK293 P2RX2(B)+P2RX3 (CVCL_D6U2)

[Text version]
Cell line name HEK293 P2RX2(B)+P2RX3
Synonyms HEK293 P2X2 B+P2X3
Accession CVCL_D6U2
Resource Identification Initiative To cite this cell line use: HEK293 P2RX2(B)+P2RX3 (RRID:CVCL_D6U2)
Comments Characteristics: Using pcDNA5/FRT/TO and pOG44, P2RX2 isoform B and P2RX3 were integrated in a single site and their expression is regulated by tetracycline.
Genetic integration: Method=Transfection; Gene=HGNC; 15459; P2RX2 (Note=Isoform B).
Genetic integration: Method=Transfection; Gene=HGNC; 8534; P2RX3.
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_U427 (Flp-In-T-REx-293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

DOI=10.3390/biom14080942
Brunings X., Schmauder R., Mrowka R., Benndorf K., Sattler C.
Subtype-specific ligand binding and activation gating in homomeric and heteromeric P2X receptors.
Biomolecules 14:942.1-942.16(2024)

Entry history
Entry creation10-Sep-2024
Last entry update10-Sep-2024
Version number1