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Cellosaurus WAe001-A-2C (CVCL_D6S8)

[Text version]
Cell line name WAe001-A-2C
Synonyms SST-P2A-mCherry; GLe002-A-2C
Accession CVCL_D6S8
Resource Identification Initiative To cite this cell line use: WAe001-A-2C (RRID:CVCL_D6S8)
Comments From: Guangzhou Laboratory; Guangzhou; China.
Characteristics: Using CRISPR/Cas9 a P2A-mCherry-3xNLS-loxP-PGKp-PuroR-LoxP construct was integrated at the C-terminus of one allele of SST (PubMed=38547668).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=38547668; DOI=10.1016/j.scr.2024.103397
Zhang T.-R., Zhang F., Wang N.-N., Xu T., Zhu L.-Q., Chen L.-H., Liu H.-S.
Generation of SST-P2A-mCherry reporter human embryonic stem cell line using the CRISPR/Cas9 system (WAe001-A-2C).
Stem Cell Res. 77:103397-103397(2024)

Cross-references
Cell line databases/resources hPSCreg; WAe001-A-2C
Biological sample resources BioSamples; SAMEA114608438
Entry history
Entry creation10-Sep-2024
Last entry update10-Sep-2024
Version number1