ID   BJ SiPS-D TH-TdTomato DJ1 HET KO
AC   CVCL_D5YD
DR   Wikidata; Q127380339
RX   PubMed=38200091;
CC   Population: Caucasian.
CC   Characteristics: Fluorescent (tdTomato) reporter cell line for TH expression. Using CRISPR/Cas9 a P2A-tdTomato construct was introduced in the C-terminal end of TH (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; 16369; PARK7 (Note=1 of 2 alleles).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
CC   Cell type: Fibroblast of foreskin; CL=CL_1001608.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C8FN ! BJ SiPS-D TH-TdTomato
SX   Male
AG   <1M
CA   Induced pluripotent stem cell
DT   Created: 02-05-24; Last updated: 10-09-24; Version: 2
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RX   PubMed=38200091; DOI=10.1038/s41467-024-44732-2; PMCID=PMC10781970;
RA   Parfitt G.M., Coccia E., Goldman C., Whitney K., Reyes R.,
RA   Sarrafha L., Nam K.H., Sohail S., Jones D.R., Crary J.F., Ordureau A.,
RA   Blanchard J., Ahfeldt T.;
RT   "Disruption of lysosomal proteolysis in astrocytes facilitates
RT   midbrain organoid proteostasis failure in an early-onset Parkinson's
RT   disease model.";
RL   Nat. Commun. 15:447.1-447.17(2024).
//