ID   4T1-Fluc-Neo (monoclonal)
AC   CVCL_D5LJ
DR   Imanis; CL126
DR   Wikidata; Q127378748
RX   PubMed=30674994;
CC   Characteristics: Reporter cell line, transfected with the LV-Fluc-P2A-Neo vector encoding for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter (from parent cell line).
CC   Characteristics: A monoclonal population was isolated using three rounds of selection in a methylcellulose-based semi-solid medium (Imanis=CL126).
CC   Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
CC   Breed/subspecies: BALB/cfC3H.
DI   NCIt; C21678; Malignant neoplasms of the mouse mammary gland
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_QZ57 ! 4T1-Fluc-Neo
SX   Female
CA   Cancer cell line
DT   Created: 02-05-24; Last updated: 10-09-24; Version: 2
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RX   PubMed=30674994; DOI=10.1038/s41417-019-0081-2; PMCID=PMC7170803;
RA   Vandergaast R., Khongwichit S., Jiang H.-L., DeGrado T.R., Peng K.-W.,
RA   Smith D.R., Russell S.J., Suksanpaisan L.;
RT   "Enhanced noninvasive imaging of oncology models using the NIS
RT   reporter gene and bioluminescence imaging.";
RL   Cancer Gene Ther. 27:179-188(2020).
//