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Cellosaurus RPMI-8226-Fluc-EmGFP (CVCL_D5KK)

[Text version]
Cell line name RPMI-8226-Fluc-EmGFP
Accession CVCL_D5KK
Resource Identification Initiative To cite this cell line use: RPMI-8226-Fluc-EmGFP (RRID:CVCL_D5KK)
Comments Population: African American.
Characteristics: Reporter cell line, transfected with the LV-SFFV-Luc2-P2A-EmGFP vector encoding for luciferase linked via a P2A cleavage site to Emerald GFP (emGFP) and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL177).
Characteristics: Produces IgG lambda (from parent cell line).
Genetic integration: Method=Transfection; Gene=FPbase; GLAD6; Emerald (Note=EmGFP, green fluorescent protein derived from GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: B-cell; CL=CL_0000236.
Sequence variations
  • Gene fusion; HGNC; 5722; IGKJ4 + HGNC; 5783; IGKV2-28; Name(s)=IGKV2-28-IGKJ4 (from parent cell line).
  • Gene fusion; HGNC; 5865; IGLJ3 + HGNC; 5888; IGLV2-14; Name(s)=IGLV2-14-IGLJ3 (from parent cell line).
  • Mutation; HGNC; 3236; EGFR; Simple; p.Thr751Ile (c.2252C>T); ClinVar=VCV000177773; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ala (c.35G>C); ClinVar=VCV000045122; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Glu285Lys (c.853G>A); ClinVar=VCV000420133; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 12033; TRAF3; Simple; p.Lys191Leufs*60; Zygosity=Unspecified (from parent cell line).
Disease Plasma cell myeloma (NCIt: C3242)
Multiple myeloma (ORDO: Orphanet_29073)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0014 (RPMI-8226)
Sex of cell Male
Age at sampling 61Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL177
Encyclopedic resources Wikidata; Q127384068
Entry history
Entry creation02-May-2024
Last entry update10-Sep-2024
Version number2