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Cellosaurus K562-Fluc-EmGFP (CVCL_D5KJ)

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Cell line name K562-Fluc-EmGFP
Accession CVCL_D5KJ
Resource Identification Initiative To cite this cell line use: K562-Fluc-EmGFP (RRID:CVCL_D5KJ)
Comments Population: Caucasian.
Characteristics: Reporter cell line, transfected with the LV-SFFV-Luc2-P2A-EmGFP vector encoding for luciferase linked via a P2A cleavage site to emerald green fluorescent protein (emGFP) and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL169).
Transfected with: UniProtKB; P42212; GFP (with p.Ser65Thr, p.Ser72Ala, p.Asn149Lys, p.Met153Thr, p.Ile167Thr and p.His231Leu = EmGFP).
Transfected with: UniProtKB; P08659; Firefly luciferase.
Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; 76; ABL1 + HGNC; 1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).
Disease Blast phase chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C9110)
Chronic myeloid leukemia (ORDO: Orphanet_521)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0004 (K-562)
Sex of cell Female
Age at sampling 53Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL169
Entry history
Entry creation02-May-2024
Last entry update02-May-2024
Version number1