Cellosaurus EMT6-eGFP-Neo/Fluc-Puro (CVCL_D5KD)
| Cell line name | EMT6-eGFP-Neo/Fluc-Puro |
|---|---|
| Accession | CVCL_D5KD |
| Resource Identification Initiative | To cite this cell line use: EMT6-eGFP-Neo/Fluc-Puro (RRID:CVCL_D5KD) |
| Comments | Characteristics: Reporter cell line, transfected with both the LV-eGFP-P2A-Neo and LV-Fluc-P2A-Puro vectors. LV-eGFP-P2A-Neo encodes for eGFP linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter and LV-Fluc-P2A-Puro encodes for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL155). Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911. |
| Disease | Malignant neoplasms of the mouse mammary gland (NCIt: C21678) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: BALB/cCRGL. |
| Hierarchy | Parent: CVCL_1923 (EMT6) |
| Sex of cell | Female |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Imanis; CL155 |
| Encyclopedic resources | Wikidata; Q127380819 |
| Entry history | |
| Entry creation | 02-May-2024 |
| Last entry update | 10-Sep-2024 |
| Version number | 2 |